A recent developed RNA extraction method can directly use culture cells as the samples. It is the use of magnetic beads covered with nucleic acid–binding matrices. Its theory is nucleic acid (DNA and RNA) in the solution is specifically adsorbed to the surface of magnetic beads because the following action: (i) shielded intermolecular electrostatic forces, (ii) dehydration of the DNA/RNA and beads surfaces, and (iii) intermolecular hydrogen bond formation in the DNA/RNA–beads contact layer.
Bionew™ cells total RNA purification kit is the excellent kit which uses magnetic beads for RNA extraction. It can be widely used in RNA purification with sample from Research Institutions, University, Life Science and Industrial Markets.
Compaing "Column method", using "Beads method" can capture RNA in more gentle operation:
No High-Speed Centrifugation to force liquid throughing the membrane to capture RNA, just capture RNA by acting the beads by vortex;
Transferring RNA on the surface of beads by place the beads on the magnetic stand to gather beads and remove beads from the magnetic stand to disperce beads, not High-Speed Centrifugation.
So, gentle operation will bring higher RNA purification efficiency for rare RNA.